Yield of nat screening in Slovenia
AbstractBackground: Safe blood supply is the main goal of blood transfusion services. Nucleic Acid Amplification Technologies (NAT) are the methods for direct detection of viral genoms in various biological samples. Introduction of NAT blood screening reduces the risk for transfusion transmitted diseases and improves the safety. Methods and Results: In Slovenia NAT screening with PCR (Polymerase Chain Reaction) method for testing pools of samples for hepatitis C virus (HCV) RNA was implemented in 2000. The screening is mandatory for all donations. Until 2007, 590,000 donated units had been screened and there were no HCV RNA-only positive result.In 2007, the testing was expanded to three viruses: HCV, hepatitis B virus (HBV) and human immunodeficiency virus (HIV). The testing method was TMA (Transcription Mediated Amplification) performed on individual donations. In five-year period, 458,000 blood units were screened and two window-period donations positive for HCV were detected (yield 1:524,000). HBV DNA-only was detected in 29 units. All the cases were donors with a resolved HBV infection, in one case the recent one, in others the occult hepatitis B infection (OBI) was confirmed. The yield of NAT testing for HBV is 1:15,600 units. There were no donations with HIV 1 RNA-only result. In all 5 HIV positive units in this period, anti-HIV antibodies were present as well. The yield of HIV NAT-only is thus 0:460,000. Conclusion: The implementation of NAT screening for three viruses has improved the blood safety. In the five-year period, 31 infectious units that were NAT–only positive were eliminated from the stock.
The Author transfers to the Publisher (Zdravniški vestnik/Slovenian Medical Journal) all economic copyrights following form Article 22 of the Slovene Copyright and Related Rights Act (ZASP), including the right of reproduction, the right of distribution, the rental right, the right of public performance, the right of public transmission, the right of public communication by means of phonograms and videograms, the right of public presentation, the right of broadcasting, the right of rebroadcasting, the right of secondary broadcasting, the right of communication to the public, the right of transformation, the right of audiovisual adaptation and all other rights of the author according to ZASP.
The aforementioned rights are transferred non-exclusively, for an unlimited number of editions, for the term of the statutory
The Author can make use of his work himself or transfer subjective rights to others only after 3 months from date of first publishing in the journal Zdravniški vestnik/Slovenian Medical Journal.
The Publisher (Zdravniški vestnik/Slovenian Medical Journal) has the right to transfer the rights, acquired parties without explicit consent of the Author.
The Author consents that the Article be published under the Creative Commons BY-NC 4.0 (attribution-non-commercial) or comparable licence.