KONFOKALNA MIKROSKOPIJA ROŽENICE
Ključne besede:
konfokalna mikroskopija, potek preiskave, morfološke spremembe v roženiciPovzetek
Izhodišča. Konfokalna mikroskopija (KM) je preiskovalna metoda, s katero pregledujemo morfološko strukturo roženice in vitro in in vivo.
Metode. S konfokalnim mikroskopom (Confoscan 2,0, Nidek, Japonska) opazujemo in analiziramo morfološke spremembe v posameznih plasteh roženice: epitelne, preko strome do endotelne plasti. Opazujemo lahko povzročitelja roženičnega vnetja in se odločamo o načinu zdravljenja ter o vrsti kirurškega posega. Raziskujemo gostoto celic v posameznih plasteh roženice: epitelnih celic, keratocitov v stromi ter endotelnih celic. Z opazovanjem gostote celic v posameznih plasteh roženice ugotavljamo celični odgovor pri poškodbah, vnetjih roženice ali po operacijah sprednjega segmenta očesa.
Zaključki. S KM opazujemo tudi neprozorno roženico, ki nastane zaradi edema epitelne plasti ali strome, česar in vivo pred odkritjem KM ni bilo mogoče.
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